A variety of approaches for nuclease cleavage detection have been developed, allowing a full-genome survey of the targeting landscape and the detection of a variety of repair outcomes for nuclease-induced double-strand breaks. 4Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, United StatesĪs genome-editing nucleases move toward broader clinical applications, the need to define the limits of their specificity and efficiency increases.3Department of Neuroscience and Center for Neurovirology, Temple University Lewis Katz School of Medicine, Philadelphia, PA, United States.2Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, United States.1Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA, United States.Nonnemacher 1,2 and Brian Wigdahl 1,2,4 * Allen 1,2 †, Will Dampier 1,2, Theodore E. Andrew Atkins 1,2, Cheng-Han Chung 1,2 †, Alexander G.
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